The standard barcoding fragment for the Barcoding of Life initiative is a ~ 640 bp long fragment located at the 5' site of the mitochondrial cytochrome oxidase subunit 1 (CO1). However, as this fragment might not display sufficient variability in sponges , we suggest sequencing an additional downstream region, which exhibits a higher substitution rate  in order to obtain a fragment of about 1,100 bp with a higher number of variable sites. A submission ITS-1 and 2 sequences, which could be added to the CO1-sequence tag, is also encouraged.
Standard barcoding primer from Folmer et al. :
LCO1490: GGT CAA CAA ATC ATA AAG ATA TTG G
HCO2198:TAA ACT TCA GGG TGA CCA AAA AAT CA
Degenerated Folmer et al.  (standard) barcoding primer designed by Meyer et al. :
dgLCO1490: GGT CAA CAA ATC ATA AAG AYA TYG G
dgHCO2198:TAA ACT TCA GGG TGA CCA AAR AAY CA
Primers from Rot et al. :
COX1-R1: TGT TGR GGG AAA AAR GTT AAA TT
COX1-D2: AAT ACT GCT TTT TTT GAT CCT GCC GG
Primers for the extended fragment, designed by Misof et al.  and Erpenbeck et al. :
CO1porF1:CCN CAN TTN KCN GMN AAA AAA CA
CO1porR1: AAN TGN TGN GGR AAR AAN G
C1Npor2760: TCT AGG TAA TCC AGC TAA ACC
C1J2165: GAA GTT TAT ATT TTA ATT TTA CCN GG
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